Assay Instruments
A number of spectrofluorometric thermal cyclers and spectrofluorometric temperature-controlled plate readers that monitor the progress of amplification reactions (i.e., Polymerase Chain Reaction (PCR), Nucleic Acid Sequence Based Amplification (NASBA), Transcription Mediated Amplification (TMA), Rolling Circle Amplification (RCA) and Strand Displacement Amplification (SDA)) by monitoring changes in fluorescence are available and are compatible with molecular beacons. In addition, fluorospectrometers are available to monitor the fluorescence generation of molecular beacons with and without nucleic acid targets present.
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Applied
Biosystems |
PRISM
7700 & 7900HT |
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bioMerieux |
NucliSens
EasyQ |
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Bio-Rad |
CFX96
& CFX384 |
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Bio-Rad |
Chromo 4 |
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Bio-Rad |
iCycler
iQ 5 |
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Bio-Rad |
MiniOpticon |
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Cepheid |
GeneXpert
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Cepheid |
SmartCycler |
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Corbett Research |
Rotor-Gene 6000 |
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Idaho
Technology |
RapidCycler 2 |
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NanoDrop
Technologies |
ND-3300 Fluorospectrometer |
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Eppendorf |
Mastercycler ep realplex |
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Roche
Applied Science |
LightCycler
1.5 and 2 |
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Roche
Applied Science |
LightCycler
480 |
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Stratagene |
Mx3000P
& Mx3005P |
Recent Publications from Our Group
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In the November 23, 2011 issue of Cell, using our unique single-molecule imaging approach, we describe circumstances under which splicing is uncoupled from transcription. An interview conducted by Cell's editorial staff with Sanjay Tyagi about this work can be listened to at www.cell.com/../paperclip.mp3 |
Vargas DY, Shah K, Batish M, Levandoski M, Sinha S, Marras SAE, Schedl P, Tyagi S (2011) Single-molecule imaging of transcriptionally coupled and uncoupled splicing Cell 147, 1054-1065.
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We describe a protocol for a novel method that utilizes single labeled Fluorescent In-situ Hybridization probes to image individual mRNA molecules |
Batish M, Raj A, and Tyagi S (2011) Single Molecule Imaging of RNA In Situ. Methods in Molecular Biology 714, 3-11.
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In collaboration with Diana Bratu and Irina Catrina at Hunter College in New York City, we published on the design parameters and applications of Locked Nucleic Acid (LNA) based molecular beacon probes to detect RNA in live cells |
Bratu DP, Catrina IE, and Marras SAE (2011) Tiny molecular beacons for in vivo mRNA detection. Methods in Molecular Biology 714, 141-157.