Assay Instruments
A number of spectrofluorometric thermal cyclers and spectrofluorometric temperature-controlled plate readers that monitor the progress of amplification reactions (i.e., Polymerase Chain Reaction (PCR), Nucleic Acid Sequence Based Amplification (NASBA), Transcription Mediated Amplification (TMA), Rolling Circle Amplification (RCA) and Strand Displacement Amplification (SDA)) by monitoring changes in fluorescence are available and are compatible with molecular beacons. In addition, fluorospectrometers are available to monitor the fluorescence generation of molecular beacons with and without nucleic acid targets present.
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Applied
Biosystems |
PRISM
7700 & 7900HT |
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bioMerieux |
NucliSens
EasyQ |
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Bio-Rad |
CFX96
& CFX384 |
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Bio-Rad |
Chromo 4 |
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Bio-Rad |
iCycler
iQ 5 |
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Bio-Rad |
MiniOpticon |
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Cepheid |
GeneXpert
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Cepheid |
SmartCycler |
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Corbett Research |
Rotor-Gene 6000 |
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Idaho
Technology |
RapidCycler 2 |
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NanoDrop
Technologies |
ND-3300 Fluorospectrometer |
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Eppendorf |
Mastercycler ep realplex |
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Roche
Applied Science |
LightCycler
1.5 and 2 |
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Roche
Applied Science |
LightCycler
480 |
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Stratagene |
Mx3000P
& Mx3005P |
Recent Publications
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In the February issue of Bioconjugate Chemistry, we report the development of luminescent probes for the detection of nucleic acids |
Krasnoperov LN, Marras SAE, Kozlov M, Wirpsza L, and Mustaev A (2010) Luminescent probes for ultrasensitive detection of nucleic acids. Bioconjugate Chemistry 21, 319 - 327.
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In collaboration with our colleagues Soumitesh Chakravorty and David Alland, we developed an assay which utilizes sloppy molecular beacon probes for the identification of more than 110 different pathogenic bacterial genotypes |
Chakravorty S, Aladegbami B, Burday M, Levi M, Marras SAE, Shah D, El-Hajj HH, Kramer FR, and Alland D (2010) Rapid universal diagnosis of bacterial pathogens from clinical
cultures using a novel sloppy molecular beacon melting temperature signature technique. Journal of Clinical Microbiology 48, 258-267.